Generator

Part:BBa_K1076004

Designed by: Carlos Gustavo Nunes   Group: iGEM13_Manaus_Amazonas-Brazil   (2013-09-13)

FadD, Long-chain-fatty-acid-CoA ligase

FadD ligates CoA in fatty acid chain preparing it to get into the Beta-Oxidation metabolic pathway. The idea is super express this protein by expressing this full lenght gene, with the promoter, rbs, gene and terminator from the proper bacteria, in order in order to produce more ATP and H+.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1934
    Illegal NgoMIV site found at 1944
    Illegal AgeI site found at 1462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 415


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Categories
Parameters
None